Annotation Detail

Information

Associated Genes: EGFR
Associated Variants: EGFR p.Leu858Arg (p.L858R) ( ENST00000275493.7, ENST00000450046.2, ENST00000455089.5 )
EGFR p.Leu858Arg (p.L858R) ( ENST00000275493.7, ENST00000450046.2, ENST00000455089.5 )
Associated Disease: cancer
Source Database: CIViC Evidence
Description: EGFR L858R was expressed in Ba/F3 cells which do not contain endogenous EGFR, and conferred growth factor independance to the cells. Cells were plated and treated with 1st generation EGFR inhibitors Gefitinib, Erlotinib, or the the tool compound AEE788, and IC50 values were measured. L858R EGFR cells had very low IC50 nmol/L values for all three inhibitors in comparison to other constructs (Gefitinib=12, Erlotinib=6, AEE788=6), indicating sensitivity to all three constructs.
Variant Origin: somatic
Variant Origin: Somatic
Evidence URL: https://civic.genome.wustl.edu/links/evidence_items/3811
Gene URL: https://civic.genome.wustl.edu/links/genes/19
Variant URL: https://civic.genome.wustl.edu/links/variants/33
Rating: 3
Evidence Type: Predictive
Disease: Cancer
Evidence Direction: Supports
Drug: Multikinase Inhibitor AEE788,Erlotinib,Gefitinib
Evidence Level: D
Clinical Significance: Sensitivity/Response
Pubmed: 19147750

Drugs